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@arup
Before I started riding fixed gear I tried leaving my geared bike in the same gear as a means of training. After a week of that my knees ached so bad that I could hardly walk up the stairs. I continued riding in the same way and the same mileage as I did before and toward the end of the second week it started to clear up.
Nowadays on fixed I tend to get the problem only if I do a lot of sprinting. Feels great at the time but I pay for it later. I have been taking cod liver oil for the last couple of years and it has certainly helped to take the edge off.
Pedaling technique helps too. I find that if I focus on a bit more on the pulling up during the pedal stroke rather than pushing down it's nicer on my knees at the end of the day.