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@AngelD
No actually, I've had the same tyre on a 16mm, 18mm, and two 23mm wide rims, and they all measured as 25mm tyre depth.
It did however change the width and air capacity of the tyre, and am now quite happily rocking two 25mm Gators at 25mm wide on the rear TB14, and 27mm wide on the front ZTR Iron Cross!