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@DFP
My fixed bike is 56cm and the seatube angled fairly back so riders weight it more over the rear.
On that bike I find riding to be more dominant on hip & outer thighs.
I also have a 52.5cm road bike with a steeper seatube and long stem so weight it more over front end. On that bike I find it utilises muscle around knee/inner thigh much much more. Im not used to using these muscles much so very quickly get a burn.