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@Ste_S
Have you tried the wheel in frame, and fits ok ? Does the dish of the wheel place the rim in the centre ? and will the chain run on the smallest sprocket without rubbing on the inside of the chain stay ? If so don't need to worry
Very roughly
120mm rear spacing - max 5 speed block
126mm rear spacing - 6 or 7 speed block
130mm rear spacing 8 speed cassete upwards