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@JDB
Hmm sounds like a hamstring injury... let me guess you do lots of cycling and not that much walking, running or hill climbing. Cycling causes hamstrings to shorten since you don't fully extend your legs in the pedal motion. Anyway you have probably just buggered the muscle, a bit of deep heat, some time and stretching should see it fix itself. Would not be suprised if my internet diagnosis is wrong, but unless you can't work because of the pain and its not located in or aroung joints its hardly worth seeking medical attention for.
Oh yeah did you do this riding or walking, its unclear, the two mile hike bit makes it sound like a walking injury in which case is probably is just hamstring strain from having funny cyclist legs.