frankenbike's frankenbikes

I thought so but I was surprised to read that some of it is muddy. And:

WHAT IS THE MOST SUITABLE BIKE TO PARTICIPATE IN THE TUSCANY TRAIL?

This is definitely one of the most frequently asked questions by participants. Let’s say right away that the track is designed to be covered by a MTB. The route is extremely varied consisting of dirt roads, asphalt secondary roads and paths. Over the years, participants in the Tuscany Trail have used all types of bike, starting from cyclocross (and gravel bikes of course) and finishing with endure bikes. The truth is that there is no single answer to this question. If you use a gravel bike, you will go very fast on the sections of dirt roads and asphalt but you will find yourself a bit in trouble along the paths and cart roads while if you use a MTB you will face all dirt roads with great ease but you will be disadvantaged on asphalt.

Definitely SPDs if I go on Checkpoint.

I think low pressure and 700c×45mm tyres should be decent