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Should be. It might not be "oh amazing" but I definitely noticed changing from 28s to 25s or 23s on the TT bike after hours training. Banging into potholes and stuff and coming back with bruised arms. Basically, there's a real difference, but how much you notice it depends on how familiar you are with your route and how much attention you're paying. I was doing the same long-ass loops every Sat and Sun so I knew all the rough spots and definitely finished rides with my head less vibrated when running the fatter tyres at lower psi.
Same thing the other week when I went from the 47mm tubeless to the 25mm road tyres. Every fucking bump tried to knock the road bike into the sky.
Quick question - for winter road riding, how much of a difference does the jump from 25mm to 28mm really make? Assuming the tyres are the same in every other way, will I notice more stability / better grip.... or not so much?