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Depends on the bag. Some are divided vertically, others horizontally, some not at all.
IME dividers are either sewn in with a separate zip for access to a lower compartment (if split horizontally), or (more commonly?) use wide velcro that overlaps to create a divider, or can be undone to allow full access to compartment.
Example:
Full framebags, do they generally have one big compartment inside, or are they subdivided, and if so, how?