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See a Physio or a bike fitter.
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The short answer is what DJ has already said.
For what it's worth my experience turned out to be that my quad wasn't firing properly making the rest of my leg take up the slack which lead to my patella not tracking correctly. A couple of weeks of physio to retrain it and all is well again.
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You've managed to write quite a lot of words, but unless I'm mistaken, not mentioned where exactly the pain is nor what type of pain it is.
dancing james
±ln(x)
hippy
@paulbiche
What could cause (I guess there are many variables) a small pain in the left knee? I does feel it a bit on the bike, and off the bike however when running it does not appear to be bothering me at all.
I am biking on a fixed gear but with a front brake so no skids at all (just slowing down with the legs complemented by the front brake) so I thought torque would be acceptable for the knees and joints
Could it be caused by bad saddle setback (but wouldn't it affect both knees?)? Could it be caused by a misalignment or bad symmetry of one component (saddle, stem, bars, etc.) even if I tried to check alignment before? Could it be caused by my saddle (not properly seated or slipping, it is a leather brooks saddle)? or maybe it is due to my pedaling technique (not pedaling straight with one leg)?
Understand it is difficult to ask it through the forum but it was just to have ideas to fuel my personal reflection