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I think I did it based on rpm so I was 80 rpm for 20 and then up to 120 for the 40. Hard to put a percentage on it at it gets harder to maintain the rpm as you approach the end of the interval. This was recommended for me when I was (trying) to train for track so something approaching 80-90% maybe cumulatively by the end?
What effort is your '40 seconds on' in % term please? Is it 100% full gas?