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It depends on how much weight I can lose beforehand but I'll probably stick to 38x25 (41"). I have a 20t rear and a 44t front I can use as well, neither of which would give me a gear I'd be happy with (maybe 44x25) so will likely use 41".
I rode Nationals back in 2008 on fixed (with double trispokes for banter) with 38x20 in Matlock and that got pretty steep in the middle! It was much shorter back then though. Will say hi if I see you, I'll be the 200kg guy trying not to get girled.
National HC now closed and with 265 entries + late callers apparently! That's the biggest entry I can remember for a while.
Probably not ideal for fixed because of the faster sections but I'm going to do it on fixed anyway, my bike is nearly <5.5kg so might as well.